Investigation of the conditioning potential of Canavalia Ensiformis (Jack bean seed)
نویسندگان
چکیده
منابع مشابه
The Globulins of the Jack Bean, Canavalia Ensiformis
While working with the urease of the jack bean, the author has had occasion to study the nature of the proteins that are present. The proteins of the jack bean have been investigated by Jones and Johns,’ who claim to have isolated an albumin and two globulins. The precipitate obtained by half saturation with ammonium sulfate, present in small amount and having the greater sulfur content, they c...
متن کاملCloning, expression, and crystallization of jack bean (Canavalia ensiformis) canavalin.
Canavalin is the major storage protein of the jack bean (Canavalia ensiformis) and belongs to the classical vicilin fraction. A full-length cDNA for canavalin was generated by the polymerase chain reaction. The nucleotide sequence coding for canavalin and the corresponding amino acid sequence were determined and shown to be homologous with those of other seed storage proteins. The amino acid se...
متن کاملImmunofluorescent localization of urease in the cotyledons of jack bean, Canavalia ensiformis.
Urease has been localized in sections of cotyledons from germinating seeds of jack bean, using FITC-labelled immunoglobulin prepared from urease antiserum raised in rabbits. The complication of lectin binding to the immunoglobulins was resolved by treatment of the sections with specific glycosides. Urease is localized in 2 sites: within the cytoplasm of storage parenchyma cells in spherical gra...
متن کاملAn Ontogenetic Study of Canavanine Formation in the Fruit of Jack Bean, Canavalia ensiformis (L.) DC.
An ontogenetic study of canavanine formation in the fruit of jack bean, Canavalia ensiformis (L.) DC. was conducted. Evidence was presented to show that the ovary wall is the reservoir for seed canavanine. The testa possesses sufficient canavanine to account for the continued elevation in seed canavanine after the pod senesces. The seed canavanine concentration is not constant inasmuch as the c...
متن کاملJack bean (Canavalia ensiformis) urease. Probing acid-base groups of the active site by pH variation.
A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in an effort to elucidate the functions exercised in the catalysis by the ionizable groups of the enzyme act...
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ژورنال
عنوان ژورنال: Global Journal of Pure and Applied Sciences
سال: 2001
ISSN: 1118-0579
DOI: 10.4314/gjpas.v7i4.16314